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Addgene

pMDR1-44
(Plasmid #69484)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69484 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2 Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5598
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MDR1 promoter
  • Alt name
    ABCB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    162
  • Mutation
    Insert includes MDR1 promoter sequence from -44 to +118
  • Entrez Gene
    ABCB1 (a.k.a. ABC20, CD243, CLCS, GP170, MDR1, P-GP, PGY1, p-170)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sma I (unknown if destroyed)
  • 3′ cloning site Cell II (unknown if destroyed)
  • 5′ sequencing primer GLprimer1
  • 3′ sequencing primer GLprimer2
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMDR1-44 was a gift from Kathleen Scotto (Addgene plasmid # 69484 ; http://n2t.net/addgene:69484 ; RRID:Addgene_69484)

  • For your References section:

    Transcriptional regulation of the MDR1 gene by histone acetyltransferase and deacetylase is mediated by NF-Y. Jin S, Scotto KW. Mol Cell Biol. 1998 Jul;18(7):4377-84. PubMed 9632821
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