pMXs-Puro-CLDN18
(Plasmid
#69466)
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PurposeExpresses CLDN18
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69466 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXs-Puro
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Backbone manufacturercell biolabs
- Backbone size w/o insert (bp) 5732
- Total vector size (bp) 6557
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Modifications to backboneAdded a gene and a HA tag
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Vector typeRetroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructions200mg/L
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCLDN18
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SpeciesH. sapiens (human)
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GenBank IDNM_016369
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Entrez GeneCLDN18 (a.k.a. SFTA5, SFTPJ)
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Tag
/ Fusion Protein
- HA TAG (N terminal on insert)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer TAGAACCTCGCTGGAAAGGA
- 3′ sequencing primer CGGGACTATGGTTGCTGACT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs-Puro-CLDN18 was a gift from Axel Hillmer (Addgene plasmid # 69466 ; http://n2t.net/addgene:69466 ; RRID:Addgene_69466) -
For your References section:
Recurrent Fusion Genes in Gastric Cancer: CLDN18-ARHGAP26 Induces Loss of Epithelial Integrity. Yao F, Kausalya JP, Sia YY, Teo AS, Lee WH, Ong AG, Zhang Z, Tan JH, Li G, Bertrand D, Liu X, Poh HM, Guan P, Zhu F, Pathiraja TN, Ariyaratne PN, Rao J, Woo XY, Cai S, Mulawadi FH, Poh WT, Veeravalli L, Chan CS, Lim SS, Leong ST, Neo SC, Choi PS, Chew EG, Nagarajan N, Jacques PE, So JB, Ruan X, Yeoh KG, Tan P, Sung WK, Hunziker W, Ruan Y, Hillmer AM. Cell Rep. 2015 Jul 14;12(2):272-85. doi: 10.1016/j.celrep.2015.06.020. Epub 2015 Jul 2. 10.1016/j.celrep.2015.06.020 PubMed 26146084