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pJBL-RNAINS4-C24AA25C
(Plasmid #69463)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69463 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    custom
  • Backbone manufacturer
    N/A
  • Backbone size w/o insert (bp) 2200
  • Total vector size (bp) 3100
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RNA-IN S4 with C24A, A25C mutations
  • Alt name
    IS10 (mutated S4 variant)
  • Species
    Synthetic
  • Insert Size (bp)
    900
  • Mutation
    C24A, A25C, and mutations for S4 variant (from wt)
  • Promoter J23119

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutated from sequence obtained from Arkin Lab (UC Berkeley)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJBL-RNAINS4-C24AA25C was a gift from Julius Lucks (Addgene plasmid # 69463 ; http://n2t.net/addgene:69463 ; RRID:Addgene_69463)

  • For your References section:

    Simultaneous characterization of cellular RNA structure and function with in-cell SHAPE-Seq. Watters KE, Abbott TR, Lucks JB. Nucleic Acids Res. 2015 Sep 8. pii: gkv879. 10.1093/nar/gkv879 PubMed 26350218
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