pattB-aubergine-ADH-gf
(Plasmid #69448)

• Purpose: genomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead Aub
• Depositing Lab: Phillip Zamore
• Publication: pattB-aubergine-WT-gf (unpublished)

Backbone

• Vector backbone: pattB
• Backbone size w/o insert (bp): 7411
• Total vector size (bp): 17232
• Vector type: Insect Expression
• Selectable markers: transgenic flies carry red eye

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: aubergine
• Species: D. melanogaster (fly)
• Insert Size (bp): 9821
• Mutation: chr2L:10,998,626(−)T>G
• Entrez Gene: aub (a.k.a. Dmel_CG6137, AUB, AUBERGINE, Aub, CG6137, Dmel\CG6137, ms(2)32D, sti, sting)
• Promoter: aubergine
• Tag / Fusion Protein:
  • NA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not I (not destroyed)
• 3′ cloning site: Not I (not destroyed)
• 5′ sequencing primer: TCC CTT AGG AGC CTC AGC AT
• 3′ sequencing primer: TGC ATA AGG TTG CCC TAC CA

Resource Information

• Supplemental Documents:
  • plasmid in snapGene format
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry