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pattB-aubergine-WT-gf
(Plasmid #69447)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69447 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pattB
  • Backbone size w/o insert (bp) 7411
  • Total vector size (bp) 17232
  • Vector type
    Insect Expression
  • Selectable markers
    transgenic flies carry red eye

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    aubergine
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    9821
  • Mutation
    many SNPs exist in the promoter region of aubergine
  • Entrez Gene
    aub (a.k.a. Dmel_CG6137, AUB, AUBERGINE, Aub, CG6137, Dmel\CG6137, ms(2)32D, sti, sting)
  • Promoter aubergine
  • Tag / Fusion Protein
    • NA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not I (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer TCC CTT AGG AGC CTC AGC AT
  • 3′ sequencing primer TGC ATA AGG TTG CCC TAC CA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pattB-aubergine-WT-gf was a gift from Phillip Zamore (Addgene plasmid # 69447 ; http://n2t.net/addgene:69447 ; RRID:Addgene_69447)
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