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Addgene

fgfprom luc
(Plasmid #69446)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69446 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL-3 basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 5005
  • Modifications to backbone
    inserted minimal fgf4 promoter upstream of luciferase gene
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    minimal fgf4 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    199

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that several discrepancies were found between Addgene's quality control and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    fgfprom luc was a gift from Lisa Dailey (Addgene plasmid # 69446 ; http://n2t.net/addgene:69446 ; RRID:Addgene_69446)

  • For your References section:

    FIREWACh: high-throughput functional detection of transcriptional regulatory modules in mammalian cells. Murtha M, Tokcaer-Keskin Z, Tang Z, Strino F, Chen X, Wang Y, Xi X, Basilico C, Brown S, Bonneau R, Kluger Y, Dailey L. Nat Methods. 2014 May;11(5):559-65. doi: 10.1038/nmeth.2885. Epub 2014 Mar 23. 10.1038/nmeth.2885 PubMed 24658142
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