6xO/S luc
(Plasmid #69445)

• Purpose: detection/assessment of OCT4 and SOX2 transcription factor activity
• Depositing Lab: Lisa Dailey
• Publication: luciferase reporter plasmid containing 6 tandem copies of the composite Oct4/Sox2 binding site of the FGF4 enhancer. Used for detection of active Oct4/Sox2 complexes (unpublished)

Backbone

• Vector backbone: PGL-3 basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Total vector size (bp): 5237
• Modifications to backbone: inserted 6XOS cassette and minimal fgf4 promoter upstream of luciferase coding sequences in PGL3 basic
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
• Species: M. musculus (mouse), Synthetic
• Insert Size (bp): 448

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that several discrepancies were found between Addgene's quality control and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.