p2,0_3E5E_eGFP
(Plasmid
#69359)
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PurposeEncodes for Ebola Minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter
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Depositing Lab
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Sequence Information
Ordering
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Availability may be limited outside the U.S. Please log in for more information.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69359 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep2,0
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Backbone manufacturerAndrew Ball, University of Alabama at Birmingham
- Backbone size w/o insert (bp) 3077
- Total vector size (bp) 5016
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Vector typeT7
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name3E5E eGFP minigenome
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Insert Size (bp)1939
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site RsRII (not destroyed)
- 3′ cloning site XmaI (not destroyed)
- 5′ sequencing primer ATT GTA TGG GAT CTG ATC TGG
- 3′ sequencing primer GCC AAC TCA GCT TCC TTT CGG (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p2,0_3E5E_eGFP was a gift from Elke Mühlberger (Addgene plasmid # 69359 ; http://n2t.net/addgene:69359 ; RRID:Addgene_69359) -
For your References section:
Recombinant Marburg virus expressing EGFP allows rapid screening of virus growth and real-time visualization of virus spread. Schmidt KM, Schumann M, Olejnik J, Krahling V, Muhlberger E. J Infect Dis. 2011 Nov;204 Suppl 3:S861-70. doi: 10.1093/infdis/jir308. 10.1093/infdis/jir308 PubMed 21987762
Map uploaded by the depositor.
