pGEX-4T1-RhoA G17A
(Plasmid #69357)

• Purpose: expression of nucleotide free RhoA in bacterial cells
• Depositing Lab: Rafael Garcia-Mata
• Publication: Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Backbone

• Vector backbone: pGEX-4T1
• Backbone manufacturer: GE
• Backbone size w/o insert (bp): 4900
• Total vector size (bp): 5500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RhoA
• Alt name: ARH12
• Alt name: ARHA
• Alt name: RHO12
• Species: H. sapiens (human)
• Insert Size (bp): 579
• Mutation: G17A
• GenBank ID: 387
• Entrez Gene: RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: pGEX3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Keith Burridge Lab, UNC Chapel Hill
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

RhoA has a mutation, G17A, which makes it nucleotide free when expressed in bacteria, which can be used GEF pulldowns.