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pGEX-4T1-Cdc42 G15A
(Plasmid #69356)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69356 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T1
  • Backbone manufacturer
    GE
  • Backbone size w/o insert (bp) 4900
  • Total vector size (bp) 5500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cdc42
  • Alt name
    G25K
  • Alt name
    Cdc42Hs
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    573
  • Mutation
    G15A
  • GenBank ID
    998
  • Entrez Gene
    CDC42 (a.k.a. CDC42Hs, G25K, TKS)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pGEX5
  • 3′ sequencing primer pGEX3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Keith Burridge Lab, UNC Chapel Hill

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cdc42 has a mutation, G15A, which makes it nucleotide free when expressed in bacteria, which can be used GEF pulldowns.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T1-Cdc42 G15A was a gift from Rafael Garcia-Mata (Addgene plasmid # 69356 ; http://n2t.net/addgene:69356 ; RRID:Addgene_69356)

  • For your References section:

    Analysis of activated GAPs and GEFs in cell lysates. Garcia-Mata R, Wennerberg K, Arthur WT, Noren NK, Ellerbroek SM, Burridge K. Methods Enzymol. 2006;406:425-37. 10.1016/S0076-6879(06)06031-9 PubMed 16472675
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