pGEX-4T1-Rac1 G15A
(Plasmid #69355)

• Purpose: expression of nucleotide free Rac1 in bacterial cells
• Depositing Lab: Rafael Garcia-Mata
• Publication: Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Backbone

• Vector backbone: pGEX-4T1
• Backbone manufacturer: GE
• Backbone size w/o insert (bp): 4900
• Total vector size (bp): 5500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rac1
• Alt name: MIG5
• Alt name: TC-25
• Alt name: p21-Rac1
• Species: H. sapiens (human)
• Insert Size (bp): 576
• Mutation: G15A
• GenBank ID: 5879
• Entrez Gene: RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: pGEX3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Keith Burridge Lab, UNC Chapel Hill

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Rac1 has a mutation, G15A, which makes it nucleotide free when expressed in bacteria, which can be used GEF pulldowns.