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Purposeexpresses mouse Nox4 in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69353 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5380
- Total vector size (bp) 1734
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemNox4
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Alt nameNox4
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Alt nameNADPH Oxydase 4
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SpeciesM. musculus (mouse)
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Insert Size (bp)1734
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GenBank ID50490
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Entrez GeneNox4
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-mNox4 was a gift from Karl-Heinz Krause (Addgene plasmid # 69353 ; http://n2t.net/addgene:69353 ; RRID:Addgene_69353) -
For your References section:
NOX4 activity is determined by mRNA levels and reveals a unique pattern of ROS generation. Serrander L, Cartier L, Bedard K, Banfi B, Lardy B, Plastre O, Sienkiewicz A, Forro L, Schlegel W, Krause KH. Biochem J. 2007 Aug 15;406(1):105-14. 10.1042/BJ20061903 PubMed 17501721