-
PurposesgRNA scaffold and synthetic U6 promoter
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69351 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneTopo Blunt II
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3500
- Total vector size (bp) 3964
-
Vector typeCRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namesU6 promoter and sgRNA scaffold flanked by BbsI sites
-
Insert Size (bp)425
- Promoter synthetic U6 promoter
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer Sp6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDonor_sU6 was a gift from Andrea Ventura (Addgene plasmid # 69351 ; http://n2t.net/addgene:69351 ; RRID:Addgene_69351) -
For your References section:
Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries. Vidigal JA, Ventura A. Nat Commun. 2015 Aug 17;6:8083. doi: 10.1038/ncomms9083. 10.1038/ncomms9083 PubMed 26278926
Map uploaded by the depositor.
