poRibo-T2
(Plasmid #69347)

• Purpose: Ribo-T with orthogonal anti-Shine-Dalgarno sequence, and pL G to T mutation
• Depositing Lab: Michael Jewett
• Publication: Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature14862.

Backbone

• Vector backbone: pAM552
• Backbone size w/o insert (bp): 3015
• Total vector size (bp): 7480
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): POP2136
• Growth instructions: Strain contains cI repressor to repress pL promoter at 30 oC.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: oRibo-T
• Insert Size (bp): 4465

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry