pT7oGFP
(Plasmid
#69346)
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PurposeOrthogonal GFP message under T7 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69346 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepT7-FLAG-4 base
- Backbone size w/o insert (bp) 5373
- Total vector size (bp) 6096
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSuper folder GFP
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Alt namesfGFP
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Insert Size (bp)723
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT7oGFP was a gift from Michael Jewett (Addgene plasmid # 69346 ; http://n2t.net/addgene:69346 ; RRID:Addgene_69346) -
For your References section:
Protein synthesis by ribosomes with tethered subunits. Orelle C, Carlson ED, Szal T, Florin T, Jewett MC, Mankin AS. Nature. 2015 Jul 29. doi: 10.1038/nature14862. 10.1038/nature14862 PubMed 26222032