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Addgene

pT7oGFP
(Plasmid #69346)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69346 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pT7-FLAG-4 base
  • Backbone size w/o insert (bp) 5373
  • Total vector size (bp) 6096
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Super folder GFP
  • Alt name
    sfGFP
  • Insert Size (bp)
    723

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7oGFP was a gift from Michael Jewett (Addgene plasmid # 69346 ; http://n2t.net/addgene:69346 ; RRID:Addgene_69346)
  • For your References section:

    Protein synthesis by ribosomes with tethered subunits. Orelle C, Carlson ED, Szal T, Florin T, Jewett MC, Mankin AS. Nature. 2015 Jul 29. doi: 10.1038/nature14862. 10.1038/nature14862 PubMed 26222032