pUA97
(Plasmid #69340)

• Purpose: It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy
• Depositing Lab: Oliver Hobert
• Publication: Hobert plasmids (unpublished)

Backbone

• Vector backbone: pUC19 (modified)
• Backbone size w/o insert (bp): 2600
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ceBFP SEC 3xFlag
• Species: C. elegans (nematode), Synthetic
• Insert Size (bp): 6628
• Mutation: sqt-1(e1350)
• Tags / Fusion Proteins:
  • ceBFP (mTagBFP)
  • 3xFlag

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pUA97 genbank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid was made based on pDD268 from Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.178335.