pBALU1
(Plasmid
#69316)
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PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of gfp
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69316 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR TOPO
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Backbone manufacturerInvitrogen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFPint-FgF
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SpeciesSynthetic
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBALU1 was a gift from Oliver Hobert (Addgene plasmid # 69316 ; http://n2t.net/addgene:69316 ; RRID:Addgene_69316) -
For your References section:
A toolkit and robust pipeline for the generation of fosmid-based reporter genes in C. elegans. Tursun B, Cochella L, Carrera I, Hobert O. PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 2009 Mar 4. 10.1371/journal.pone.0004625 PubMed 19259264
