pBS-UPRT
(Plasmid
#69310)
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Purposeexpresses UPRT cDNA
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69310 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBScri
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Backbone manufacturerAgilent Technologies
- Backbone size w/o insert (bp) 2900
- Total vector size (bp) 3691
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Modifications to backbonenone
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUPRT cDNA
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SpeciesToxoplasma gondii
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Insert Size (bp)791
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr Mike Cleary
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-UPRT was a gift from Chris Doe (Addgene plasmid # 69310 ; http://n2t.net/addgene:69310 ; RRID:Addgene_69310) -
For your References section:
Applying thiouracil tagging to mouse transcriptome analysis. Gay L, Karfilis KV, Miller MR, Doe CQ, Stankunas K. Nat Protoc. 2014 Feb;9(2):410-20. doi: 10.1038/nprot.2014.023. Epub 2014 Jan 23. 10.1038/nprot.2014.023 PubMed 24457332