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Addgene

pYPQ152
(Plasmid #69303)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69303 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ185-linker1
  • Backbone manufacturer
    (modified from pNJB185)
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pco-dCas9-VP64 (Plant codon-optimized)
  • Mutation
    VP64 activator fusion; NLS fusion

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site AatII (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ152 was a gift from Yiping Qi (Addgene plasmid # 69303 ; http://n2t.net/addgene:69303 ; RRID:Addgene_69303)

  • For your References section:

    A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, Zheng X, Hsieh TF, Qi Y. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015. 10.1104/pp.15.00636 PubMed 26297141
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