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Addgene

pYPQ151
(Plasmid #69302)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69302 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    NJB91
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pcoCas9D10A (Plant codon-optimized)
  • Mutation
    D10A mutation; NLS fusion
  • Tag / Fusion Protein
    • 2XFLAG (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ151 was a gift from Yiping Qi (Addgene plasmid # 69302 ; http://n2t.net/addgene:69302 ; RRID:Addgene_69302)

  • For your References section:

    A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, Zheng X, Hsieh TF, Qi Y. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015. 10.1104/pp.15.00636 PubMed 26297141
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