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Purpose(Empty Backbone) Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69296 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYPQ104
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the Addgene verified sequence has minor differences compared to the depositor's reference sequence, however these differences should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQ144 was a gift from Yiping Qi (Addgene plasmid # 69296 ; http://n2t.net/addgene:69296 ; RRID:Addgene_69296) -
For your References section:
A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, Zheng X, Hsieh TF, Qi Y. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015. 10.1104/pp.15.00636 PubMed 26297141