pSR49
(Plasmid
#69259)
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Purposephlh-8::tagBFP2
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69259 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPD49_26
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Backbone manufacturerAndrew Fire
- Backbone size w/o insert (bp) 3417
- Total vector size (bp) 4710
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Vector typeWorm Expression, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTagBFP2
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SpeciesC. elegans (nematode)
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Insert Size (bp)804
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Mutationcodon-optimzed index 1.0, TagBFP2 contains 2 single nucleotide changes compared to TagBFP
- Promoter hlh-8
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSR49 was a gift from Sander van den Heuvel (Addgene plasmid # 69259 ; http://n2t.net/addgene:69259 ; RRID:Addgene_69259) -
For your References section:
G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit during Muscle Differentiation. Ruijtenberg S, van den Heuvel S. Cell. 2015 Jul 16;162(2):300-13. doi: 10.1016/j.cell.2015.06.013. Epub 2015 Jul 2. 10.1016/j.cell.2015.06.013 PubMed 26144318
Map uploaded by the depositor.
