pGL3 RFPL4b promoter
(Plasmid #69250)

• Purpose: Luciferase reporter for Dux4 activation of RFPL4b transcription
• Depositing Lab: Stephen Tapscott
• Publication: Dux4 reporters (unpublished)

Backbone

• Vector backbone: pGL3 basic
• Backbone manufacturer: promega
• Backbone size w/o insert (bp): 4818
• Total vector size (bp): 5480
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RFPL4b
• Species: H. sapiens (human)
• Insert Size (bp): 662
• Entrez Gene: RFPL4B (a.k.a. RNF211)
• Promoter: RFPL4b

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: sacI (not destroyed)
• 5′ sequencing primer: RVp3
• 3′ sequencing primer: GLp2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

related information can be found in:
DUX4 activates germline genes, retroelements, and immune mediators: implications for facioscapulohumeral dystrophy.
Geng LN, Yao Z, Snider L, Fong AP, Cech JN, Young JM, van der Maarel SM, Ruzzo WL, Gentleman RC, Tawil R, Tapscott SJ.
Dev Cell. 2012 Jan 17;22(1):38-51. doi: 10.1016/j.devcel.2011.11.013. Epub 2011 Dec 29.
PMID: 22209328