pRP-iSL(MCS1+2)
(Plasmid
#69245)
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Purpose(Empty Backbone) Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a random RRNA spacer locus in T. brucei.
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69245 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneDerived from pLEW100 (Wirtz et al, 1999) and p2T7TAblue (Alibu et al., 2005).
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Vector typeexpression in Trypanosoma brucei
- Promoter RRNA promoter
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRP-iSL(MCS1+2) was a gift from David Horn (Addgene plasmid # 69245 ; http://n2t.net/addgene:69245 ; RRID:Addgene_69245)