pRPa-iSL(MCS1+2)
(Plasmid
#69244)
-
Purpose(Empty Backbone) Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69244 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneDerived from pLEW100 (Wirtz et al, 1999) and p2T7TAblue (Alibu et al., 2005).
-
Vector typeexpression in Trypanosoma brucei
- Promoter RRNA promoter
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRPa-iSL(MCS1+2) was a gift from David Horn (Addgene plasmid # 69244 ; http://n2t.net/addgene:69244 ; RRID:Addgene_69244) -
For your References section:
Single-locus targeting constructs for reliable regulated RNAi and transgene expression in Trypanosoma brucei. Alsford S, Horn D. Mol Biochem Parasitol. 2008 Sep;161(1):76-9. doi: 10.1016/j.molbiopara.2008.05.006. Epub 2008 Jun 7. 10.1016/j.molbiopara.2008.05.006 PubMed 18588918
