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PurposeRFP-Atg8 (PAS marker) with improved property
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69201 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneRS404
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDuDre-Atg8
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)6800
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Tag
/ Fusion Protein
- DsRed Express 2 (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer yCUP1pro-Fwd
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PCUP1-DuDre-Atg8-404 was a gift from Zhiping Xie (Addgene plasmid # 69201 ; http://n2t.net/addgene:69201 ; RRID:Addgene_69201) -
For your References section:
A fluorescent tool set for yeast Atg proteins. Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, Xie Z. Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971. 10.1080/15548627.2015.1040971 PubMed 25998947
