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Addgene

BS-Trp1
(Plasmid #69196)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69196 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Bluescript II SK+
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRP1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    5300

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Although this is the plasmid described in the associated publication, the depositor notes that it has a low integration efficiency in yeast and Plasmid 69503 should be used.

Please note there are a few discrepancies between the QC and full sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BS-Trp1 was a gift from Zhiping Xie (Addgene plasmid # 69196 ; http://n2t.net/addgene:69196 ; RRID:Addgene_69196)

  • For your References section:

    A fluorescent tool set for yeast Atg proteins. Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, Xie Z. Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971. 10.1080/15548627.2015.1040971 PubMed 25998947
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