LD229
(Plasmid #69192)

• Purpose: Integration of 2GFP tag at Atg29 C terminus, use auxotrophic marker URA3(Candida albicans)
• Depositing Lab: Zhiping Xie
• Publication: Li et al Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.

Backbone

• Vector backbone: Bluescript II SK+
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Atg29-2EGFP
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 5400
• Tag / Fusion Protein:
  • 2xEGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry