LD217
(Plasmid
#69186)
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PurposeIntegration of 2GFP tag at Atg17 C terminus, use auxotrophic marker URA3(Candida albicans)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69186 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBluescript II SK+
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAtg17-2EGFP
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)4900
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Tag
/ Fusion Protein
- 2xEGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LD217 was a gift from Zhiping Xie (Addgene plasmid # 69186 ; http://n2t.net/addgene:69186 ; RRID:Addgene_69186) -
For your References section:
A fluorescent tool set for yeast Atg proteins. Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, Xie Z. Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971. 10.1080/15548627.2015.1040971 PubMed 25998947
