pNeoXTR f2
(Plasmid
#69159)
-
Purposemouse targeting vector
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 69159 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBS
-
Backbone manufacturerAgilent
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 7776
-
Modifications to backbonesee pNeoXTR f2.gb file
-
Vector typeMouse Targeting
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Stbl3
-
Copy numberUnknown
Gene/Insert 1
-
Gene/Insert nameDTA
-
Speciesdiptheria
-
Insert Size (bp)984
Gene/Insert 2
-
Gene/Insert nameNeoR
-
Insert Size (bp)804
Gene/Insert 3
-
Gene/Insert nameGFP
-
Alt namegreen fluorescent protein
-
Insert Size (bp)720
Gene/Insert 4
-
Gene/Insert nameAmpR
-
Insert Size (bp)861
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pNeoXTR f2 was a gift from David Feldser (Addgene plasmid # 69159 ; http://n2t.net/addgene:69159 ; RRID:Addgene_69159) -
For your References section:
Recombinase-based conditional and reversible gene regulation via XTR alleles. Robles-Oteiza C, Taylor S, Yates T, Cicchini M, Lauderback B, Cashman CR, Burds AA, Winslow MM, Jacks T, Feldser DM. Nat Commun. 2015 Nov 5;6:8783. doi: 10.1038/ncomms9783. 10.1038/ncomms9783 PubMed 26537451