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Addgene

pNeoXTR f2
(Plasmid #69159)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69159 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBS
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 7776
  • Modifications to backbone
    see pNeoXTR f2.gb file
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    DTA
  • Species
    diptheria
  • Insert Size (bp)
    984

Gene/Insert 2

  • Gene/Insert name
    NeoR
  • Insert Size (bp)
    804

Gene/Insert 3

  • Gene/Insert name
    GFP
  • Alt name
    green fluorescent protein
  • Insert Size (bp)
    720

Gene/Insert 4

  • Gene/Insert name
    AmpR
  • Insert Size (bp)
    861

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNeoXTR f2 was a gift from David Feldser (Addgene plasmid # 69159 ; http://n2t.net/addgene:69159 ; RRID:Addgene_69159)
  • For your References section:

    Recombinase-based conditional and reversible gene regulation via XTR alleles. Robles-Oteiza C, Taylor S, Yates T, Cicchini M, Lauderback B, Cashman CR, Burds AA, Winslow MM, Jacks T, Feldser DM. Nat Commun. 2015 Nov 5;6:8783. doi: 10.1038/ncomms9783. 10.1038/ncomms9783 PubMed 26537451
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