pNeoXTR f0
(Plasmid #69157)

• Purpose: mouse targeting vector
• Depositing Lab: David Feldser
• Publication: Robles-Oteiza et al Nat Commun. 2015 Nov 5;6:8783. doi: 10.1038/ncomms9783.

Backbone

• Vector backbone: pBS
• Backbone manufacturer: Agilent
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 7775
• Modifications to backbone: see pNeoXTR f0.gb file
• Vector type: Mouse Targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: DTA
• Species: diptheria
• Insert Size (bp): 984
• Promoter: PGK

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: tctaccgggtaggggaggcg

Gene/Insert 2

• Gene/Insert name: NeoR
• Insert Size (bp): 804
• Promoter: PGK

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: tctaccgggtaggggaggcg

Gene/Insert 3

• Gene/Insert name: GFP
• Alt name: green fluorescent protein
• Insert Size (bp): 720
• Promoter: none

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: ttacttgtacagctcgtcca

Gene/Insert 4

• Gene/Insert name: AmpR
• Insert Size (bp): 861
• Promoter: Amp promoter

Cloning Information for Gene/Insert 4

• Cloning method: Unknown
• 5′ sequencing primer: tctaccgggtaggggaggcg

Resource Information

• Supplemental Documents:
  • pNeoXTR f0.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry