pSR13
(Plasmid #69155)

• Purpose: read-outloxN mCherry to GFP switch, with swsn-1 promoter, gene (partially cDNA) and UTR, for integration on on ttTi5605, Mos Chr II
• Depositing Lab: Sander van den Heuvel
• Publication: Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10.1016/j.cell.2015.06.013. Epub 2015 Jul 2.

Backbone

• Vector backbone: pCFJ350
• Backbone manufacturer: Erik Jorgensen
• Backbone size w/o insert (bp): 7517
• Total vector size (bp): 16904
• Vector type: Worm Expression, Cre/Lox ; MosSCI

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mCherry
• Species: C. elegans (nematode)
• Insert Size (bp): 861
• Mutation: codon-optimzed index 1.0
• Promoter: rps-27

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13 reverse
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: swsn-1
• Species: C. elegans (nematode)
• Insert Size (bp): 6058
• Mutation: first exon plus intron, then cDNA
• Entrez Gene: swsn-1 (a.k.a. CELE_Y113G7B.23)
• Promoter: swsn-1

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 3

• Gene/Insert name: eGFP
• Species: C. elegans (nematode)
• Insert Size (bp): 869
• Mutation: codon-optimzed index 1.0, A to T mutation at bp 793 in GFP, GFP still visible but possibly weaker

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

discrepancies between QC and full sequence should not have functional consequences