pSR04
(Plasmid #69151)

• Purpose: read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV
• Depositing Lab: Sander van den Heuvel
• Publication: Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10.1016/j.cell.2015.06.013. Epub 2015 Jul 2.

Backbone

• Vector backbone: pCFJ356
• Backbone manufacturer: Erik Jorgensen
• Backbone size w/o insert (bp): 7606
• Total vector size (bp): 12662
• Vector type: Worm Expression, Cre/Lox ; MosSCI

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mCherry
• Species: C. elegans (nematode)
• Insert Size (bp): 861
• Mutation: codon-optimzed index 1.0
• Promoter: rps-27

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13 reverse
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: TagBFP
• Species: C. elegans (nematode)
• Insert Size (bp): 804
• Mutation: codon-optimzed index 1.0
• Promoter: eef-1A.1 (eft-3)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 3

• Gene/Insert name: eGFP
• Species: C. elegans (nematode)
• Insert Size (bp): 869
• Mutation: codon-optimzed index 1.0

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3