SGL40C.EFS.RFP657
(Plasmid #69147)

• Purpose: Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with RFP657 expression (EFS Promoter)
• Depositing Lab: Dirk Heckl
• Publication: Reimer et al Haematologica. 2017 Jun 1. pii: haematol.2017.164046. doi: 10.3324/haematol.2017.164046.

Backbone

• Vector backbone: SIN40C
• Modifications to backbone: modified pLKO5

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: sgRNA
• gRNA/shRNA sequence: nt
• Insert Size (bp): 75

Gene/Insert 2

• Gene/Insert name: EFS
• Alt name: short EF1-alpha promoter
• gRNA/shRNA sequence: na
• Insert Size (bp): 250

Gene/Insert 3

• Gene/Insert name: tagRFP657
• gRNA/shRNA sequence: na
• Species: Synthetic
• Insert Size (bp): 700

Resource Information

• Supplemental Documents:
  • Genbank pSGL40C-NL-SL.EFS.tRFP657
  • DH_CRISPR-Cas9 spacer cloning protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry