L40C-CRISPR.EFS.mNeon
(Plasmid
#69146)
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PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter driven
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69146 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSIN40C
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Modifications to backbonemodified pLKO5
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Vector typeLentiviral, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameSpCas9
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gRNA/shRNA sequencent
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SpeciesS. pyogenes
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Insert Size (bp)4300
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Gene/Insert 2
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Gene/Insert nameSp sgRNA scaffold
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gRNA/shRNA sequencent
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Insert Size (bp)75
Gene/Insert 3
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Gene/Insert nameEFS
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Alt nameshort EF1alpha Promoter
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gRNA/shRNA sequencena
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SpeciesH. sapiens (human)
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Insert Size (bp)250
Gene/Insert 4
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Gene/Insert nameP2A-mNeonGreen
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gRNA/shRNA sequencena
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SpeciesSynthetic
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Insert Size (bp)750
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
L40C-CRISPR.EFS.mNeon was a gift from Dirk Heckl (Addgene plasmid # 69146 ; http://n2t.net/addgene:69146 ; RRID:Addgene_69146) -
For your References section:
CRISPR-Cas9-induced t(11;19)/MLL-ENL translocations initiate leukemia in human hematopoietic progenitor cells in vivo. Reimer J, Knoess S, Labuhn M, Charpentier EM, Gohring G, Schlegelberger B, Klusmann JH, Heckl D. Haematologica. 2017 Jun 1. pii: haematol.2017.164046. doi: 10.3324/haematol.2017.164046. 10.3324/haematol.2017.164046 PubMed 28572162