L40C-CRISPR.EFS.mNeon
(Plasmid #69146)

• Purpose: Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter driven
• Depositing Lab: Dirk Heckl
• Publication: Reimer et al Haematologica. 2017 Jun 1. pii: haematol.2017.164046. doi: 10.3324/haematol.2017.164046.

Backbone

• Vector backbone: SIN40C
• Modifications to backbone: modified pLKO5
• Vector type: Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: SpCas9
• gRNA/shRNA sequence: nt
• Species: S. pyogenes
• Insert Size (bp): 4300
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Gene/Insert 2

• Gene/Insert name: Sp sgRNA scaffold
• gRNA/shRNA sequence: nt
• Insert Size (bp): 75

Gene/Insert 3

• Gene/Insert name: EFS
• Alt name: short EF1alpha Promoter
• gRNA/shRNA sequence: na
• Species: H. sapiens (human)
• Insert Size (bp): 250
• Entrez Gene: EEF1A1 (a.k.a. CCS-3, CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, EF1A1, EF1alpha1, GRAF-1EF, LENG7, PTI1, eEF1A-1)

Gene/Insert 4

• Gene/Insert name: P2A-mNeonGreen
• gRNA/shRNA sequence: na
• Species: Synthetic
• Insert Size (bp): 750

Resource Information

• Supplemental Documents:
  • pL40C-CRISPR.EFS.mNeon NL SL.gb
  • DH_CRISPR-Cas9 spacer cloning protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry