pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)
(Plasmid #69138)

• Purpose: For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo
• Depositing Lab: Takuji Iwasato
• Publication: Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 10.1016/j.neuron.2014.02.026. Epub 2014 Mar 27.

Backbone

• Vector backbone: pDisplay
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4339
• Total vector size (bp): 10266
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
• Alt name: CAG-LSL-RFP-ires-tTA-WPRE
• Species: G. gallus (chicken); CMV, P1 phage, SV40 , Entacmaea quadricolor, Encephalomyocarditis virus, E.coli, HSV, woodchuck hepatitis virus
• Insert Size (bp): 5927
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry