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PurposeFor the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69136 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTRE-tight
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 2546
- Total vector size (bp) 3684
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNLS-Cre
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SpeciesSV40 , P1 phage
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Insert Size (bp)1138
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pCEP_fwd_primer (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pK031.TRE-Cre (Supernova) was a gift from Takuji Iwasato (Addgene plasmid # 69136 ; http://n2t.net/addgene:69136 ; RRID:Addgene_69136) -
For your References section:
NMDAR-regulated dynamics of layer 4 neuronal dendrites during thalamocortical reorganization in neonates. Mizuno H, Luo W, Tarusawa E, Saito YM, Sato T, Yoshimura Y, Itohara S, Iwasato T. Neuron. 2014 Apr 16;82(2):365-79. doi: 10.1016/j.neuron.2014.02.026. Epub 2014 Mar 27. 10.1016/j.neuron.2014.02.026 PubMed 24685175