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Addgene

pTM1_NP_ZEBOV
(Plasmid #69121)

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Item Catalog # Description Quantity Price (USD)
Plasmid 69121 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTM1
  • Backbone manufacturer
    Bernhard Moss, National Institutes of Health
  • Backbone size w/o insert (bp) 5357
  • Total vector size (bp) 7977
  • Vector type
    T7

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NP ZEBOV
  • Insert Size (bp)
    2629
  • Entrez Gene
    NP (a.k.a. ZEBOVgp1)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site StuI (destroyed during cloning)
  • 5′ sequencing primer ATT GTA TGG GAT CTG ATC TGG
  • 3′ sequencing primer GCC AAC TCA GCT TCC TTT CGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTM1_NP_ZEBOV was a gift from Elke Mühlberger (Addgene plasmid # 69121 ; http://n2t.net/addgene:69121 ; RRID:Addgene_69121)

  • For your References section:

    The L-VP35 and L-L interaction domains reside in the amino terminus of the Ebola virus L protein and are potential targets for antivirals. Trunschke M, Conrad D, Enterlein S, Olejnik J, Brauburger K, Muhlberger E. Virology. 2013 Jul 5;441(2):135-45. doi: 10.1016/j.virol.2013.03.013. Epub 2013 Apr 11. 10.1016/j.virol.2013.03.013 PubMed 23582637
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