pBrain-GFP-TACC3KDP(L566,567A)-shTACC3
(Plasmid #69117)

• Purpose: Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
• Depositing Lab: Stephen Royle
• Publication: Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843.

Backbone

• Vector backbone: pBrain
• Backbone size w/o insert (bp): 5438
• Total vector size (bp): 7952
• Vector type: Mammalian Expression, RNAi
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Transforming Acidic Coiled Coil protein 3
• Alt name: TACC3
• Alt name: ERIC1
• Species: H. sapiens (human)
• Insert Size (bp): 2514
• Mutation: LL (566-567aa) mutated to AA. Silent mutations in amino acids 30-33 to confer siRNA resistance.
• GenBank ID: NM_006342.2
• Entrez Gene: TACC3 (a.k.a. ERIC-1, ERIC1, Tacc4, maskin)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Gene/Insert 2

• Gene/Insert name: shTACC3
• Species: H. sapiens (human)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Human TACC3 Clone: IMAGE ID 6148176 (Clone BC106071).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry