pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG
(Plasmid #69114)

• Purpose: Dual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.
• Depositing Lab: Stephen Royle
• Publication: Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843.

Backbone

• Vector backbone: pBrain
• Backbone size w/o insert (bp): 5447
• Total vector size (bp): 11543
• Vector type: Mammalian Expression, RNAi
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Colonic Hepatic Tumour Over-expressed Gene
• Alt name: ch-TOG
• Alt name: CKAP5
• Species: H. sapiens (human)
• Insert Size (bp): 6096
• Mutation: L1939A and L1942A amino acid substitutions. Silent mutations to confer shRNA resistance.
• GenBank ID: NM_001008938
• Entrez Gene: CKAP5 (a.k.a. CHTOG, MSPS, TOG, TOGp, ch-TOG)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: AfeI (not destroyed)
• 3′ cloning site: DraIII (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Gene/Insert 2

• Gene/Insert name: shch-TOG
• Species: H. sapiens (human)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Human ch-TOG purchased. IMAGE ID: 40072423 (Clone BC120869).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry