His6-PRC1
(Plasmid #69111)

• Purpose: For bacterial expression of recombinant human PRC1 tagged with His.
• Depositing Lab: Stephen Royle
• Publication: Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635.

Backbone

• Vector backbone: pRSET-B
• Backbone size w/o insert (bp): 2803
• Total vector size (bp): 4666
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Protein Regulator of Cytokinesis 1
• Alt name: PRC1
• Alt name: ASE1
• Alt name: MAP65
• Species: H. sapiens (human)
• Insert Size (bp): 1863
• Entrez Gene: PRC1 (a.k.a. ASE1, MAP65)
• Tag / Fusion Protein:
  • His6 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Human PRC1 purchased as IMAGE clone (reference unknown).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry