MBP-ch-TOG-His6
(Plasmid #69108)

• Purpose: For bacterial expression of recombinant human ch-TOG tagged with MBP and His.
• Depositing Lab: Stephen Royle
• Publication: Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635.

Backbone

• Vector backbone: pMALPre-His
• Backbone size w/o insert (bp): 6669
• Total vector size (bp): 12767
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Colonic Hepatic Tumour Over-expressed Gene
• Alt name: ch-TOG
• Alt name: CKAP5
• Species: H. sapiens (human)
• Insert Size (bp): 6098
• GenBank ID: NM_001008938
• Entrez Gene: CKAP5 (a.k.a. CHTOG, MSPS, TOG, TOGp, ch-TOG)
• Tags / Fusion Proteins:
  • MBP (N terminal on backbone)
  • His6 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EagI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ch-TOG cDNA purchased. IMAGE ID: 40072423 (Clone BC120869).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry