GST-TPX2(1-43)
(Plasmid #69107)

• Purpose: For bacterial expression of human TPX2(1-43aa) tagged with GST.
• Depositing Lab: Stephen Royle
• Publication: Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635.

Backbone

• Vector backbone: pGEX-6p-1
• Backbone size w/o insert (bp): 4981
• Total vector size (bp): 5128
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Targeting Protein for Xklp2
• Alt name: TPX2, microtubule-associated
• Species: H. sapiens (human)
• Mutation: Amino acids 1-43 expressed.
• Entrez Gene: TPX2 (a.k.a. C20orf1, C20orf2, DIL-2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, REPP86, p100)
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Insert amplified from IMAGE clone (ID unknown).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry