pRS416-yZ3EV-Z3pr-yEGFP (RB3579)
(Plasmid #69100)

• Purpose: URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP
• Depositing Lab: David Botstein
• Publication: pRS416-yZ3EV-Z3pr (unpublished)

Backbone

• Vector backbone: pRS416
• Total vector size (bp): 7796
• Modifications to backbone: Addition of yZ3EV cassette and Z3pr; CYC1 3'UTR is still there from the parent vector (pRS416GPD)
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: yZ3EV cassette
• Species: Synthetic
• Insert Size (bp): 2021

Gene/Insert 2

• Gene/Insert name: Z3 promoter
• Species: Synthetic
• Insert Size (bp): 699

Gene/Insert 3

• Gene/Insert name: yEGFP
• Species: Synthetic
• Insert Size (bp): 717

Resource Information

• Supplemental Documents:
  • RB3579 Genbank file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry