pMJ915
(Plasmid #69090)

• Purpose: Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS
• Depositing Lab: Jennifer Doudna
• Publication: Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.7554/eLife.04766.

Backbone

• Vector backbone: pML-2CT
• Backbone manufacturer: MacroLab, UC Berkeley
• Backbone size w/o insert (bp): 5950
• Total vector size (bp): 10094
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cas9
• Species: Streptococcus pyogenes
• Insert Size (bp): 5358
• Promoter: T7
• Tags / Fusion Proteins:
  • MBP (N terminal on backbone)
  • hexa His (N terminal on backbone)
  • two SV40 NLS (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: Martin Jinek at University of Zurich, Switzerland
• Articles Citing this Plasmid:
  • 21 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry