pLPC-L647RLaminA-CS
(Plasmid #69062)

• Purpose: encodes a lamin A mutant with Leucine 647 mutated to an Arginin and with a substitution of cysteine in CaaX-motif by a serine
• Depositing Lab: Gerardo Ferbeyre
• Publication: Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. doi: 10.1080/15384101.2015.1053671. Epub 2015 Jun 1.

Backbone

• Vector backbone: pLPC
• Backbone size w/o insert (bp): 5493
• Total vector size (bp): 7488
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LaminA
• Species: H. sapiens (human)
• Insert Size (bp): 1995
• Mutation: Lamin A with Leucine 647 mutated to Arginine and with substitution of cysteine in CaaX-motif by serine
• Entrez Gene: LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: taggcgtgtacggtggga
• 3′ sequencing primer: acctgtaggtttggcaag

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry