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Addgene

pcDNA3.1_PsChR2_EYFP
(Plasmid #69057)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69057 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PsChR2
  • Alt name
    Previously designated "PsChR"
  • Species
    Platymonas subcordiformis
  • Insert Size (bp)
    1714
  • Promoter CMV
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1_PsChR2_EYFP was a gift from John Spudich (Addgene plasmid # 69057 ; http://n2t.net/addgene:69057 ; RRID:Addgene_69057)

  • For your References section:

    Characterization of a highly efficient blue-shifted channelrhodopsin from the marine alga Platymonas subcordiformis. Govorunova EG, Sineshchekov OA, Li H, Janz R, Spudich JL. J Biol Chem. 2013 Oct 11;288(41):29911-22. doi: 10.1074/jbc.M113.505495. Epub 2013 Aug 30. 10.1074/jbc.M113.505495 PubMed 23995841
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