plenti-CAG-IKZF3-Q147H-FLAG-IRES-GFP
(Plasmid
#69050)
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Purposelentiviral expression of IKZF3 with Q147H mutation and FLAG tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69050 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneplenti-CAG -IRES-GFP
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIKZF3
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Alt nameIKAROS family zinc finger 3
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SpeciesH. sapiens (human)
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MutationQ147H
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Entrez GeneIKZF3 (a.k.a. AIO, AIOLOS, IMD84, ZNFN1A3)
- Promoter CAG
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Tags
/ Fusion Proteins
- FLAG (C terminal on insert)
- IRES-GFP (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pCAG-F
- 3′ sequencing primer pCDH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
plenti-CAG-IKZF3-Q147H-FLAG-IRES-GFP was a gift from William Kaelin (Addgene plasmid # 69050 ; http://n2t.net/addgene:69050 ; RRID:Addgene_69050) -
For your References section:
The myeloma drug lenalidomide promotes the cereblon-dependent destruction of Ikaros proteins. Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK, Bradner JE, Kaelin WG Jr. Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. Epub 2013 Nov 29. 10.1126/science.1244917 PubMed 24292623