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pEF6-CSPG4-myc-his
(Plasmid #69037)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69037 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF6-Myc-His-B
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 5802
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CSPG4
  • Alt name
    MCSP; NG2; HMW-MAA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6966
  • Mutation
    none
  • GenBank ID
    1464
  • Entrez Gene
    CSPG4 (a.k.a. CSPG4A, HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2)
  • Promoter EF-1 alpha
  • Tags / Fusion Proteins
    • Myc (C terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Spe I (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BstX I, EcoRI, and EcoRV sites were destroyed during cloning

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF6-CSPG4-myc-his was a gift from Wensheng Wei (Addgene plasmid # 69037 ; http://n2t.net/addgene:69037 ; RRID:Addgene_69037)

  • For your References section:

    Chondroitin sulfate proteoglycan 4 functions as the cellular receptor for Clostridium difficile toxin B. Yuan P, Zhang H, Cai C, Zhu S, Zhou Y, Yang X, He R, Li C, Guo S, Li S, Huang T, Perez-Cordon G, Feng H, Wei W. Cell Res. 2015 Feb;25(2):157-68. doi: 10.1038/cr.2014.169. Epub 2014 Dec 30. 10.1038/cr.2014.169 PubMed 25547119
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