pES004 (pTet-qacR 5)
(Plasmid #69033)

• Purpose: qacR 5 downstream of a Tet promoter
• Depositing Lab: Richard Murray
• Publication: de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Backbone

• Vector backbone: p15A Ori/cm resistant
• Backbone manufacturer: unknown
• Backbone size w/o insert (bp): 1154
• Total vector size (bp): 3117
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alphaZ1
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: qacR
• Species: Synthetic
• Insert Size (bp): 596
• Mutation: W61Y, E90Q, F102S, M116Q, Y119L, E120Q
• Entrez Gene: qacR (a.k.a. pTZ2162_28)
• Promoter: pTet
• Tag / Fusion Protein:
  • His6 tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: GCTTATCATCGATAAGCTTCC
• 3′ sequencing primer: CGCCCGGTAGTGATCTTAT

Resource Information

• Supplemental Documents:
  • pTet-qacR 5

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry